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There is a dramatic increase in cell proliferation in the inter papilla region with addition I-BET-762 of EGF in culture. Further, EGF can block the effect of Shh signal disruption, to double quantity of fungiform papillae. Together our data assistance the hypothesis that EGF/EGFR activation leads to elevated cell cycle progression whilst inhibiting differentiation to a papilla pathway; this would prevent formation of fungiform papillae and therefore reduce papilla number. From our prior studies we understand that the inter papilla epithelium is competent to type fungiform papillae . For that reason, we had proposed that regulatory components need to act directly or through other signaling components to suppress fungiform papilla formation and enable patterned spacing of papillae.
Our current data offer strong evidence for EGF/EGFR signaling in suppressing papilla formation in element by sustaining cell proliferation amongst papillae. EGF in development of epithelial specializations: feather, hair and denticle EGF and EGFR are in chick embryo skin prior to feather placodes type, after which are reduced in placodes but maintained I-BET-762 in the inter bud epidermis . In culture EGF stimulates epidermal proliferation and expands inter bud EGFR gene expression, with a concurrent loss of feather bud gene expression. Conversely, EGFR inhibitors result in loss of inter bud fate and lead to feather bud fusion. In hair follicles, EGFR is absent from epidermal cells over dermal condensates that mark the first stage of follicle development . EGF inhibits formation of hair buds in embryonic mouse skin culture .
In transgenic mice that constitutively express EGF in skin, hair follicle development is retarded in postnatal animals and also the epidermis is thickened . General, reports suggest that EGFR directs epidermal cells to an inter feather or interfollicle fate, whereas inhibition of EGFR leads to feather or hair follicle differentiation. In Drosophila epidermis, belts of hair like denticles alternate with smooth cuticle. Decreased EGFR signaling increases inter denticle apoptosis and leads to fusion of adjacent denticle belts , indicating a conserved effect of EGF in epidermal organ formation. Distributions and effects of EGF/EGFR signaling in the tongue epithelium during papilla development are equivalent to those in skin and outer cuticle, during feather, hair follicle and denticle formation.
EGFR expression is in inter papilla epithelium, and activation with EGF results in elevated cell proliferation amongst papillae; this leads to expansion of interpapilla space and loss of papillae. EGFR inhibition induces elevated number and fusion of papillae. Our data add the taste papilla as an epithelial specialization that relies on EGF/ EGFR signaling for patterning, and demonstrates common EGF/EGFR effects in creating tongue epithelium, an oral mucosa, compared to skin. Intracellular pathways and synergistic roles in EGF/EGFR signaling EGF/EGFR signaling results in simultaneous activation of many intracellular pathways, which is often functionally linked . We studied PI3K/Akt, MEK/ERK, and p38 MAPK in papilla development, pathways widely related with cell survival, proliferation, differentiation, migration and death that are preferentially activated in response to growth components or cell anxiety .
Signaling in tongue cultures—We detected phosphorylated Akt, ERK1/2, and p38 MAPK in lingual epithelium of non treated E14+2 day cultures with immunohistochemistry and Western blots, suggesting active endogenous signaling in embryonic tongue. With EGF in tongue culture medium, immunoproducts of phosphorylated Akt, ERK1/2, or p38 MAPK had been much more intense in the epithelium compared to controls, implicating all three signaling cascades in the EGF effect on fungiform papilla development. Increased kinase intensity was particularly pronounced in inter papilla epithelium, consistent with expression of EGFR in this location.
In assistance of data from immunoreactions, in Western blot assays exogenous EGF effected a dramatic increase in levels of phosphorylated Akt and ERK1/2 in the epithelium of E14+2 day cultures. Further, when a specific inhibitor for each kinase was employed , Akt and ERK1/2 phosphorylation was totally blocked with out adjust in total kinase level. However, no substantial adjust in phosphorylated p38 MAPK was observed in Western blots, in contrast to elevated lingual immunoproducts of phosphorylated p38 MAPK. Additionally, when SB203580 was employed to block signaling via p38 MAPK, the phosphorylation of p38 MAPK was not inhibited in Western blot analysis. This is equivalent to reports demonstrating that SB203580 inhibits activity of p38 MAPK by blocking activation of downstream components, but not the activation/phosphorylation of p38 MAPK itself . SB203580 inhibits p38 and B splice variants of p38 MAPK ; p38 reportedly is the most physiologically crucial variant, but p38B has suggested roles in defending against apoptosis . Clearly p38 MAPK pathways are complex and further experiment